Yeast Saccharomyces Cerevisiae

Saccharomyces cerevisiae, commonly called baker’s or brewer’s yeast, is a unicellular fungus that serves as both a biotechnology workhorse and a powerful model organism in biology. It reproduces primarily by budding and obtains energy through glycolysis, while under low-oxygen conditions it ferments sugars into ethanol and carbon dioxide. These properties support bread making, brewing, biofuel production, and other industrial processes. Researchers also use S. cerevisiae to investigate conserved eukaryotic processes, including cell-cycle regulation, gene expression, metabolism, and responses to environmental stress, providing insights relevant to human health and fundamental cell biology.

Yeast Saccharomyces Cerevisiae - Related Videos

Education

JoVE Science Education - Basic Biology

An Introduction to Saccharomyces cerevisiae

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2023

Saccharomyces cerevisiae (commonly known as baker’s yeast) is a single-celled eukaryote that is frequently used in scientific research. S. cerevisiae is an attractive model organism due to the fact that its genome has been sequenced, its genetics are easily manipulated, and it is very easy to maintain in the lab. Because many yeast proteins are similar in sequence and function to those found in other organisms, studies performed in yeast can help us to determine how a particular gene or...

Research

JoVE Journal - Biology
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A Fluorescence Microscopy Assay for Monitoring Mitophagy in the Yeast Saccharomyces cerevisiae

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Cited by 26 •

2011

A robust approach to monitor the delivery of organelles to the acidic lumen of the yeast vacuole for degradation and recycling is described. The method relies on the specific labeling of target organelles with a genetically encoded dual-emission fluorescence pH-biosensor, and visualization of individual cells using fluorescence microscopy.

Cycloheximide Chase Analysis of Protein Degradation in Saccharomyces cerevisiae

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Cited by 70 •

2016

Protein abundance reflects the rates of both protein synthesis and protein degradation. This article describes the use of cycloheximide chase followed by western blotting to analyze protein degradation in the model unicellular eukaryote, Saccharomyces cerevisiae (budding yeast).

Efficient Sporulation of Saccharomyces cerevisiae in a 96 Multiwell Format

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Cited by 3 •

2016

Here, sporulation of Saccharomyces cerevisiae is carried out in a 96 multiwell format.

Directed Evolution Method in Saccharomyces cerevisiae: Mutant Library Creation and Screening

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Cited by 10 •

2016

We present a detailed protocol to construct and screen mutant libraries for directed evolution campaigns in Saccharomyces cerevisiae.

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