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JoVE Journal
Developmental Biology

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Genotyping and Quantification of In Situ Hybridization Staining in Zebrafish
 

Genotyping and Quantification of In Situ Hybridization Staining in Zebrafish

Article doi: 10.3791/59956
January 28th, 2020 Usage Statistics

Summary January 28th, 2020

Gene editing technologies have enabled researchers to generate zebrafish mutants to investigate gene function with relative ease. Here, we provide a guide to perform parallel embryo genotyping and quantification of in situ hybridization signals in zebrafish. This unbiased approach provides greater accuracy in phenotypical analyses based on in situ hybridization.

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