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Genotyping and Quantification of In Situ Hybridization Staining in Zebrafish
JoVE Journal
Developmental Biology
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JoVE Journal Developmental Biology
Genotyping and Quantification of In Situ Hybridization Staining in Zebrafish

Genotyping and Quantification of In Situ Hybridization Staining in Zebrafish

DOI:
10.3791/59956-v

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05:41 min

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January 28, 2020

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, ,
1MRC Molecular Haematology Unit, MRC Weatherall Institute of Molecular Medicine, John Radcliffe Hospital,University of Oxford, 2BHF Centre of Research Excellence, 3Institute of Cancer and Genomic Sciences,University of Birmingham

Chapters

  • 00:04Title
  • 00:40ISH-stained Embryo Imaging and DNA Extraction
  • 02:26Image Analysis with Fiji Software
  • 03:53Results: Parallel Image Quantitation and Genotyping Protocol
  • 05:13Conclusion

Summary

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Gene editing technologies have enabled researchers to generate zebrafish mutants to investigate gene function with relative ease. Here, we provide a guide to perform parallel embryo genotyping and quantification of in situ hybridization signals in zebrafish. This unbiased approach provides greater accuracy in phenotypical analyses based on in situ hybridization.

Tags

Keywords: GenotypingIn Situ HybridizationQuantificationImage AnalysisZebrafishGene ExpressionDNA ExtractionAlkaline LysisROIImage ProcessingFiji

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