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Serum Free Production of Three-dimensional Human Hepatospheres from Pluripotent Stem Cells
This content is Free Access.
1MRC Centre for Regenerative Medicine,University of Edinburgh, 2UCL Great Ormond Street Institute of Child Health,University College London, 3Division of Biosciences, Department of Life Sciences, College of Health and Life Sciences,Brunel University London, 4School of Biochemistry and Immunology, Trinity Biomedical Sciences Institute,Trinity College Dublin
Chapters
- 00:04Title
- 00:31Preparation of Agarose Microplate Molds
- 01:12Seeding Human Pluripotent Stem Cells into Agarose Microwell Plates
- 03:07Differentiating hPSCs to 3D Hepatospheres
- 05:28Results: Hepatosphere Structure and Function
- 06:16Conclusion
This protocol describes an approach to produce hepatospheres from human pluripotent stem cells using a defined culture system and cell self-assembly. This protocol is reproducible in a number of cell lines, cost effective and allows the production of stable human hepatospheres for biomedical application.
