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JoVE Journal
Developmental Biology

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Serum Free Production of Three-dimensional Human Hepatospheres from Pluripotent Stem Cells
 

Serum Free Production of Three-dimensional Human Hepatospheres from Pluripotent Stem Cells

Article doi: 10.3791/59965
July 20th, 2019

Summary July 20th, 2019

This protocol describes an approach to produce hepatospheres from human pluripotent stem cells using a defined culture system and cell self-assembly. This protocol is reproducible in a number of cell lines, cost effective and allows the production of stable human hepatospheres for biomedical application.

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