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Anti-RDL and Anti-mGlutR1 Receptors Antibody Testing in Honeybee Brain Sections using CRISPR-Cas9
JoVE Journal
Neuroscience
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JoVE Journal Neuroscience
Anti-RDL and Anti-mGlutR1 Receptors Antibody Testing in Honeybee Brain Sections using CRISPR-Cas9

Anti-RDL and Anti-mGlutR1 Receptors Antibody Testing in Honeybee Brain Sections using CRISPR-Cas9

DOI:
10.3791/59993-v

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09:25 min

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January 30, 2020

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, , , , , , , ,
1Department of Neuroscience,University of Arizona, 2School of Life Sciences,Arizona State University, 3Department of Scientific and Technologic Investigations,University of Sonora, 4Integrated DNA Technologies, Inc.

Chapters

  • 00:05Introduction
  • 01:02Resistance to Dieldrin (RDL) and Metabotropic Glutamate Receptor 1 (mGlutR1) Protein Expression
  • 03:15Injection Procedure
  • 05:03Brain Tissue Harvest and Sectioning
  • 07:11Results: Representative Anti-RDL and Anti-mGlutR1 Receptor Antibody Testing in Honeybee Brain Sections
  • 08:44Conclusion

Summary

Automatic Translation

Automatic Translation

Presented here is a protocol to use the CRISPR-Cas9 system for reducing the production of a protein in the adult honeybee brain to test antibody specificity.

Tags

Keywords: CRISPR-Cas9Gene EditingHoneybee BrainIonotropic GABAA ReceptorsMetabotropic Glutamate ReceptorsRDLMGlutR1Immunocytochemical AnalysisGuide RNARibonucleoprotein ComplexCas9 Nuclease

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