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Journal / Biochemistry / Kinetisk screening af Nukleaseaktivitet ved hjælp …
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Biochemistry

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JoVE Journal Biochemistry
Kinetisk screening af Nukleaseaktivitet ved hjælp af nukleinsyre sonder
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Kinetisk screening af Nukleaseaktivitet ved hjælp af nukleinsyre sonder

Article DOI: 10.3791/60005-v • 06:52 min • November 1st, 2019
November 1st, 2019 •
*1,2,3, *1,2,3, 1,3, 1,3, 1,2,3, 1,2,3
1Department of Physics, Chemistry and Biology, Linköping University, 2Wallenberg Centre for Molecular Medicine (WCMM), 3Nucleic Acids Technologies Laboratory (NAT-lab), Linköping University
* These authors contributed equally

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Ændret nukleaseaktivitet har været forbundet med forskellige menneskelige forhold, underliggende dets potentiale som en biomarkør. Den modulære og nem at implementere screeningmetode præsenteret i dette papir giver mulighed for udvælgelse af specifikke nukleinsyre sonder til at udnytte nuclease aktivitet som en biomarkør af sygdom.

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Biokemi screening metode nukleaser biomarkører nukleinsyrer sonder nukleaseaktivitet diagnostisk værktøj substrat
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