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Hybrid Microdrive System with Recoverable Opto-Silicon Probe and Tetrode for Dual-Site High Density Recording in Freely Moving Mice
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Neuroscience
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JoVE Journal Neuroscience
Hybrid Microdrive System with Recoverable Opto-Silicon Probe and Tetrode for Dual-Site High Density Recording in Freely Moving Mice

Hybrid Microdrive System with Recoverable Opto-Silicon Probe and Tetrode for Dual-Site High Density Recording in Freely Moving Mice

DOI:
10.3791/60028-v

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08:57 min

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August 10, 2019

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, ,
1Department of Psychiatry,University of Texas Southwestern Medical Center, 2Department of Neuroscience,University of Texas Southwestern Medical Center

Chapters

  • 00:04Title
  • 00:54Microdrive Array Parts and Microdrive Screw Preparation
  • 02:33Shuttle Preparation
  • 03:08Optorode Preparation
  • 06:43Results: Representative Simultaneous Tetrode/Silicon-Probe Stimulation and Recording
  • 08:08Conclusion

Summary

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This protocol describes the construction of a hybrid microdrive array that allows implantation of nine independently adjustable tetrodes and one adjustable opto-silicon probe in two brain regions in freely moving mice. Also demonstrated is a method for safely recovering and reusing the opto-silicon probe for multiple purposes.

Tags

Keywords: Hybrid MicrodriveOpto-silicon ProbeTetrodeDual-site High Density RecordingFreely Moving MiceSilicon ProbeOptical FiberMicrodrive Array3D PrintingDental AcrylicPEEKEpoxyShuttle Assembly

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