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Quantification of Protein Interaction Network Dynamics using Multiplexed Co-Immunoprecipitation
JoVE Journal
Biochemistry
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JoVE Journal Biochemistry
Quantification of Protein Interaction Network Dynamics using Multiplexed Co-Immunoprecipitation

Quantification of Protein Interaction Network Dynamics using Multiplexed Co-Immunoprecipitation

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00:07 min

August 21, 2019

DOI:

00:07 min
August 21, 2019

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Summary

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Quantitative Multiplex Immunoprecipitation (QMI) uses flow cytometry for sensitive detection of differences in the abundance of targeted protein-protein interactions between two samples. QMI can be performed using a small amount of biomaterial, does not require genetically engineered tags, and can be adapted for any previously defined protein interaction network.

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