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A Method to Study de novo Formation of Chromatin Domains
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Genetics
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JoVE Journal Genetics
A Method to Study de novo Formation of Chromatin Domains

A Method to Study de novo Formation of Chromatin Domains

DOI:
10.3791/60039-v

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00:07 min

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August 23, 2019

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,
1Howard Hughes Medical Institute,New York University School of Medicine, 2Department of Biochemistry and Molecular Pharmacology,New York University School of Medicine, 3Whitehead Institute for Biomedical Research

Chapters

  • 00:04Title
  • 00:37Generation of Clonal EED Knockout Mouse Embryonic Stem Cells
  • 02:51Engineering Cre-ERT2 Based Inducible EED Expression
  • 04:11Following Nucleation and Spreading of PRC2 Activity on Chromatin
  • 05:10Results: Validations and Representative Applications of Inducible EED Rescue Systems
  • 06:43Conclusion

Summary

Automatic Translation

Automatic Translation

This method is designed to follow formation of PRC2-mediated chromatin domains in cell lines, and the method can be adapted to many other systems.

Tags

Keywords: Chromatin DomainsPRC2CRISPREEDMouse Embryonic Stem CellsGenotypingDonor Template DNA

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