JoVE
JoVE
Faculty Resource Center
Research
Behavior
Biochemistry
Biology
Bioengineering
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
JoVE Journal
JoVE Encyclopedia of Experiments
JoVE Chrome Extension
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to your Course
Authors
Librarians
High Schools
About
Sign-In
Sign In
Contact Us
Research
JoVE Journal
JoVE Encyclopedia of Experiments
Education
JoVE Core
JoVE Science Education
JoVE Lab Manual
High Schools
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Close
Research
Behavior
Biochemistry
Bioengineering
Biology
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
Products
JoVE Journal
JoVE Encyclopedia of Experiments
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
Products
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to Your Course
Teacher Resources
Get in Touch
Instant Trial
Log In
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Journal
/
Developmental Biology
/
小鼠原发性颅神经冠细胞的解剖、培养与分析,用于神经冠细胞脱角和迁移研究
JoVE Journal
Developmental Biology
This content is Free Access.
JoVE Journal
Developmental Biology
Dissection, Culture and Analysis of Primary Cranial Neural Crest Cells from Mouse for the Study of Neural Crest Cell Delamination and Migration
Please note that all translations are automatically generated.
Click here for the English version.
小鼠原发性颅神经冠细胞的解剖、培养与分析,用于神经冠细胞脱角和迁移研究
DOI:
10.3791/60051-v
•
09:33 min
•
October 03, 2019
•
Sandra Guadalupe Gonzalez Malagon*
1,2
,
Lisa Dobson*
1,3
,
Anna M Lopez Muñoz
,
Marcus Dawson
,
William Barrell
3
,
Petros Marangos
4
,
Matthias Krause
,
Karen J Liu
1
Centre for Craniofacial and Regenerative Biology
,
King’s College London
,
2
Institute of Molecular Biology and Biotechnology, FORTH, Department of Biomedical Research
,
University of Ioannina
,
3
Randall Centre of Cell & Molecular Biophysics
,
King’s College London
,
4
Department of Biological Applications and Technology
,
University of Ioannina
Chapters
00:04
Title
01:05
Early Somite Stage Embryo Dissection (Day 1)
03:12
Live Cell Imaging of Murine Cranial Neural Crest Cells (Day 2)
04:36
Neural Crest Single Cell Tracking
05:22
Quantification of Neural Crest Cell Migratory Capacity
06:04
Quantification of Neural Crest Cell Area and Circularity
07:00
Results: Representative Cranial Neural Crest Cell Migration and Cell Shape Dynamics Analyses
08:43
Conclusion
Summary
Automatic Translation
English (Original)
العربية (Arabic)
中文 (Chinese)
Nederlands (Dutch)
français (French)
Deutsch (German)
עברית (Hebrew)
italiano (Italian)
日本語 (Japanese)
한국어 (Korean)
português (Portuguese)
русский (Russian)
español (Spanish)
Türkçe (Turkish)
Automatic Translation
该协议描述了从小鼠模型颅神经峰细胞的解剖和培养,主要用于研究细胞迁移。我们描述了使用的实时成像技术,并分析了速度和细胞形状的变化。
Tags
Primary Cranial Neural Crest Cells
Neural Crest Cell Delamination
Neural Crest Cell Migration
Neural Plate
Embryonic Dissection
Neural Crest Development
Mouse Embryo
Cell Culture
Developmental Biology
Article
Embed
ADD TO PLAYLIST
Usage Statistics
Related Videos
使用小鸡
GFP
神经管移植和碳水化合物染料 DiI 注射,在鸡胚胎内对神经囊细胞和血管进行双重标记
小鼠胚胎干细胞的无血清单层培养神经细胞分化
培养人多能性和神经干细胞的基本和临床前研究一个封闭的细胞培养系统
多光子时间推移成像实时可视化发展: 迁移神经嵴细胞在斑马鱼胚胎中的可视化
人脑神经前体细胞神经发育表型的快速检测 (npc)
人胎脑神经干细胞 Zika 病毒感染的免疫细胞化学分析
从外周单核细胞生成诱导神经干细胞,分化至多巴胺能神经元前体,用于移植研究
利用人类胚胎干细胞的单细胞培养进行高效神经分化
神经冠状原脂肪衍生干细胞的分离、培养和异位诱导,来自围阴脂肪组织
小鼠胚胎干细胞中神经祖细胞和神经元的分化与表征
Read Article