Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Biochemistry / SUMO-Binding Entities (SUBEs) as Tools for the…
JoVE Journal
Biochemistry

This content is Open Access.

JoVE Journal Biochemistry
SUMO-Binding Entities (SUBEs) as Tools for the Enrichment, Isolation, Identification, and Characterization of the SUMO Proteome in Liver Cancer
Isolation of Intermediate Filament Proteins from Multiple Mouse Tissues to Study Aging-associated Post-translational Modifications
Journal (Biochemistry)
Isolation of Intermediate Filament Proteins from Multiple Mouse Tissues to Study Aging-associated Post-translational Modifications
Measuring Protein Binding to F-actin by Co-sedimentation
Journal (Biochemistry)
Measuring Protein Binding to F-actin by Co-sedimentation
 

SUMO-Binding Entities (SUBEs) as Tools for the Enrichment, Isolation, Identification, and Characterization of the SUMO Proteome in Liver Cancer

Article DOI: 10.3791/60098-v • 08:29 min • November 1st, 2019
November 1st, 2019 •
1, 1, 1, 2, 2, 3, 1
1Liver Disease and Liver Metabolism Lab, CIC bioGUNE, Centro de Investigación Biomédica en Red de Enfermedades Hepáticas y Digestivas (CIBERehd), 2Proteomics Platforms, CIC bioGUNE, ProteoRED-ISCIII, CIBERehd, 3Advanced Technology Institute in Life Sciences (ITAV)-CNRS-IPBS, UbiCARE

Chapters

Summary
Automatic Translation

Here, we present a protocol to enrich, isolate, identify, and characterize proteins modified by SUMO in vivo both from human hepatoma cells and liver tumors obtained from mouse models of hepatocellular carcinoma by using SUMO-binding entities (SUBEs).

Tags

SUMO-binding Entities SUBEs Enrichment Isolation Identification Characterization SUMO Proteome Liver Cancer SUMOylated Proteins SUMO-specific Protease Affinity Tandem Repeats Of SIMs Modified Proteins Fast And Sensitive Method Therapeutic Approach Tissues Human Specimens Animal Models Cellular Models
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter