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A 3D Spheroid Model as a More Physiological System for Cancer-Associated Fibroblasts Differentiation and Invasion In Vitro Studies
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Cancer Research
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JoVE Journal Cancer Research
A 3D Spheroid Model as a More Physiological System for Cancer-Associated Fibroblasts Differentiation and Invasion In Vitro Studies

A 3D Spheroid Model as a More Physiological System for Cancer-Associated Fibroblasts Differentiation and Invasion In Vitro Studies

DOI:
10.3791/60122-v

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06:27 min

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August 08, 2019

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,
1Institute of Physiological Chemistry and Pathobiochemistry,University of Münster, 2Instituto de Medicina Molecular, Lisbon

Chapters

  • 00:04Title
  • 00:413D Spheroids as an In Vitro Model for Fibroblast/CAF Differentiation Using Different TGF-β1 Inhibiting Compounds of Cell-matrix Interaction
  • 02:02Immunofluorescent Staining of Spheroids
  • 04:44Results: A 3D Spheroid Model for Cancer-Associated Fibroblasts Differentiation and Invasion
  • 05:37Conclusion

Summary

Automatic Translation

Automatic Translation

The goal of this protocol is to establish a 3D in vitro model to study the differentiation of cancer-associated fibroblasts (CAFs) in a tumor bulk-like environment, which can be addressed in different analysis systems, such as immunofluorescence, transcriptional analysis and life cell imaging.

Tags

Keywords: 3D Spheroid ModelCancer-associated FibroblastsFibroblast DifferentiationTumor-like EnvironmentCell Matrix InteractionMethylcelluloseSpheroid FormationTGF-betaCell CultureIn Vitro Study

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