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特定神经元种群与圆虫卡埃纳哈布迪炎的分离

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Developmental Biology

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JoVE Journal Developmental Biology

Isolation of Specific Neuron Populations from Roundworm Caenorhabditis elegans

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特定神经元种群与圆虫卡埃纳哈布迪炎的分离

DOI:

10.3791/60145-v

•

09:42 min

•

August 06, 2019

•

Edward M. Germany, Nataliya Zahayko, Oleh Khalimonchuk^2,3,4

¹Department of Biochemistry,University of Nebraska, ²Nebraska Redox Biology Center,University of Nebraska, ³Nebraska Center for Integrated Biomolecular Communication,University of Nebraska, ⁴Fred & Pamela Buffett Cancer Center,University of Nebraska Medical Center

Chapters

00:04Title
00:32Cuticle Disruption and Single Cell Isolation
03:22Isolation of GFP-positive Cells via Flow Cytometry
04:20Extraction of RNA from Isolated Cells
06:55Results: Isolation of Specific Neuron Populations
08:40Conclusion

Summary

Automatic Translation

在这里,我们提出了一个方案,用于简单分离特定组活神经元细胞表达绿色荧光蛋白从转基因开诺哈布迪炎叶兰根线。这种方法使各种外体研究侧重于特定的神经元,并有能力分离细胞,以进一步的短期培养。

Tags

C. Elegans Neuron Isolation Cell Culture Transcriptional Response SDS-DTT Isolation Buffer Protease Cell Dissociation Leibovitz’s L-15 Medium Cell Pelleting Cell Washing

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