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Differentiation of Mouse Breast Epithelial HC11 and EpH4 Cells
 

Differentiation of Mouse Breast Epithelial HC11 and EpH4 Cells

Article DOI: 10.3791/60147
February 27th, 2020

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Summary February 27th, 2020

We describe techniques for differentiation induction of two breast epithelial lines, HC11 and EpH4. While both require fetal calf serum, insulin, and prolactin to produce milk proteins, EpH4 cells can fully differentiate into mammospheres in three-dimensional culture. These complementary models are useful for signal transduction studies of differentiation and neoplasia.

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