Mouse Breast Epithelial Cells
Mouse Breast Epithelial Cells - Related Videos
11:47
Time-lapse Imaging of Primary Preneoplastic Mammary Epithelial Cells Derived from Genetically Engineered Mouse Models of Breast Cancer
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Cited by 4 •
2013
Time-lapse imaging is used to assess behavior of primary preneoplastic mammary epithelial cells derived from genetically engineered mouse models of breast cancer risk to determine if there are correlations between specific behavioral parameters and distinct genetic lesions.
08:13
Live-imaging of Breast Epithelial Cell Migration After the Transient Depletion of TIP60
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Cited by 3 •
2017
Here, we present the real-time monitoring of cell migration in a wound-healing assay using TIP60-depleted MCF10A breast epithelial cells. The implementation of live-cell imaging techniques in our protocol allows us to analyze and visualize single-cell movement in real time and across time.
09:32
Differentiation of Mouse Breast Epithelial HC11 and EpH4 Cells
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Cited by 6 •
2020
We describe techniques for differentiation induction of two breast epithelial lines, HC11 and EpH4. While both require fetal calf serum, insulin, and prolactin to produce milk proteins, EpH4 cells can fully differentiate into mammospheres in three-dimensional culture. These complementary models are useful for signal transduction studies of differentiation and neoplasia.
07:13
Initiation of Metastatic Breast Carcinoma by Targeting of the Ductal Epithelium with Adenovirus-Cre: A Novel Transgenic Mouse Model of Breast Cancer
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Cited by 27 •
2014
Activation of latent mutations with adenovirus-Cre into the mammary ductal system results in a clinically relevant metastatic breast cancer. Incorporation of a YFP promoter allows tracking of distal metastatic tumor cells. This model is useful to study latent metastasis, anti-tumor immunity, and for designing novel immunotherapies to treat breast cancer.
08:37
Isolation of Mouse Endometrial Epithelial and Stromal Cells for In Vitro Decidualization
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Cited by 86 •
2017
This study presents a standardized and validated method for the isolation and culture of primary mouse endometrial stromal and epithelial cells, which can be used in a coculture system to study in vitro decidualization.
FAQs
Apical-basal polarity organizes epithelial cells according to their tissue-facing and tissue-supporting surfaces, while cell-cell junctions maintain coordinated contact between neighboring cells. Together, these features provide reference points for assessing whether proliferation and differentiation remain regulated. Changes in this organization can therefore signal altered tissue behavior during cellular transformation and cancer-related investigation.
Proliferation controls how cell populations expand, whereas differentiation reflects how cells acquire specialized tissue characteristics. Mammary tissue signals regulate both processes, making them useful indicators of normal versus altered cellular behavior. When transformation disrupts these controls, investigators can examine how oncogene activity may change tissue organization, growth patterns, and progression toward cancer-related phenotypes.
Primary mouse breast epithelial cells and established cell lines allow investigators to examine related questions in different experimental contexts. Primary cells support analysis of epithelial behavior closer to the tissue source, while established lines provide a controlled system for repeated studies. Comparing results across both types can clarify whether findings reflect general mechanisms or model-specific behavior.
Mouse origin supports genetic manipulation and allows cellular experiments to be connected with engineered tumor models. This combination helps investigators move from observing altered epithelial behavior to examining how specific genetic changes relate to tumor development. The resulting link between controlled cell studies and disease models can reveal mechanisms relevant to cancer biology and therapeutic research.
These cells can support studies of oncogene activity, tumor initiation, invasion, and treatment response under controlled conditions. Investigators can ask how altered signaling or regulation affects epithelial organization, proliferation, and differentiation, then assess consequences for cancer-associated behavior. This breadth makes the model useful for connecting early cellular changes with later disease-related processes.
A study can use primary cells or established cell lines to examine a defined cancer-related process, such as oncogene activity, invasion, or response to treatment. Findings from these controlled experiments can then be integrated with engineered tumor models to relate cellular behavior to tumor development and to identify mechanisms for further therapeutic investigation.