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从人类母乳中分离白细胞，用于HIV靶点抗体依赖性细胞噬菌体测定
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Immunology and Infection
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JoVE Journal Immunology and Infection
Isolation of Leukocytes from Human Breast Milk for Use in an Antibody-dependent Cellular Phagocytosis Assay of HIV Targets
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从人类母乳中分离白细胞，用于HIV靶点抗体依赖性细胞噬菌体测定

DOI:
10.3791/60149-v

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08:12 min

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September 06, 2019

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,
1Division of Infectious Diseases,Icahn School of Medicine at Mount Sinai

Chapters

  • 00:04Title
  • 00:44Target Microsphere Preparation
  • 02:20Antibody-Dependent Cellular Phagocytosis (ADCP) Assay Plate Preparation
  • 02:53Breast Milk Cell Isolation
  • 03:48ADCP Assay
  • 05:11Flow Cytometric Analysis
  • 06:21Results: Representative Flow Cytometric and ADCP Analyses of HIV Targets
  • 07:42Conclusion

Summary

Automatic Translation

Automatic Translation

母乳传播人体免疫缺陷病毒（HIV），尽管只有15%的感染艾滋病毒的母亲母乳喂养的婴儿被感染。母乳喂养的婴儿每天摄入+105+108母性白细胞，尽管这些细胞研究不足。在这里，我们描述了乳腺癌白细胞的分离，并分析了它们的噬菌体能力。

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