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Encapsulated Cell Technology for the Delivery of Biologics to the Mouse Eye
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Bioengineering
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JoVE Journal Bioengineering
Encapsulated Cell Technology for the Delivery of Biologics to the Mouse Eye

Encapsulated Cell Technology for the Delivery of Biologics to the Mouse Eye

DOI:
10.3791/60162-v

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06:10 min

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March 30, 2020

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, , , , ,
1Department of Cell Biology,University of South Carolina, 2Department of Ophthalmology, Division of Research,Medical University of South Carolina, 3Department of Neuroscience, Division of Research,Medical University of South Carolina, 4Division of Research,Ralph H. Johnson VA Medical Center

Chapters

  • 00:04Introduction
  • 00:51Cell Culture and Encapsulation
  • 02:49Confirmation of Cell Viability and Capsule Size
  • 04:56Results: Cell Encapsulation and Delivery into the Eye
  • 05:32Conclusion

Summary

Automatic Translation

Automatic Translation

Presented here is a protocol for the use of alginate as a polymer in microencapsulation of immortalized cells for long-term delivery of biologics to rodent eyes.

Tags

Keywords: Encapsulated Cell TechnologyBiologics DeliveryMouse EyeAlginate MicrocapsulesRetinal Epithelial CellsCell EncapsulationSustained Drug DeliveryLIVE/DEAD AssayCell Viability

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