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DOI: 10.3791/60162-v
Marwa Belhaj1, Balasubramaniam Annamalai2, Nathaniel Parsons2, Andrew Shuler1, Jay Potts1, Bärbel Rohrer2,3,4
1Department of Cell Biology,University of South Carolina, 2Department of Ophthalmology, Division of Research,Medical University of South Carolina, 3Department of Neuroscience, Division of Research,Medical University of South Carolina, 4Division of Research,Ralph H. Johnson VA Medical Center
Presented here is a protocol for the use of alginate as a polymer in microencapsulation of immortalized cells for long-term delivery of biologics to rodent eyes.
This technique is a dependable way to deliver any content such as drugs, proteins, or cells to specific locations in tissues or animals. You can track the delivery and tune the release of the contents to fit the experiment you desire. It can be used to treat numerous diseases from wound healing to ocular and cardiac diseases.
The main advantage of this method is the ability to deliver therapeutic cells using alginate microcapsules to the target sites without causing any immune reactions and maintain a sustained delivery of fresh secreted therapeutics for a longer period of time. Begin by culturing retinal epithelial cells according to manuscript directions. Passage the cells when they reach 70 to 80%confluency using standard tissue culture procedures.
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