Brightfield Imaging
Brightfield Imaging - Related Videos
12:54
Simultaneous Brightfield, Fluorescence, and Optical Coherence Tomographic Imaging of Contracting Cardiac Trabeculae Ex Vivo
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Cited by 5 •
2021
This protocol presents a collection of sarcomere, calcium, and macroscopic geometric data from an actively contracting cardiac trabecula ex vivo. These simultaneous measurements are made possible by the integration of three imaging modalities.
14:29
Capturing Tissue Repair in Zebrafish Larvae with Time-lapse Brightfield Stereomicroscopy
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Cited by 27 •
2015
We present a protocol for capturing the dynamics of zebrafish larval tail fin regeneration on a whole-tissue scale using brightfield-based stereomicroscopy. This technique enables capturing the regeneration dynamics with single cell resolution. This methodology can be adapted to any stereomicroscope equipped with a CCD camera and time-lapse software.
07:35
Quantifying Liver Size in Larval Zebrafish Using Brightfield Microscopy
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Cited by 8 •
2020
Here we demonstrate a method for quantifying liver size in larval zebrafish, providing a way to assess the effects of genetic and pharmacologic manipulations on liver growth and development.
13:45
Hybrid µCT-FMT imaging and image analysis
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Cited by 34 •
2015
We describe a protocol for hybrid imaging, combining fluorescence-mediated tomography (FMT) with micro computed tomography (µCT). After fusion and reconstruction, we perform interactive organ segmentation to extract quantitative measurements of the fluorescence distribution.
09:24
Molecular Imaging to Target Transplanted Muscle Progenitor Cells
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Cited by 3 •
2013
A non-invasive means to evaluate the success of myoblast transplantation is described. The method takes advantage of a unified fusion reporter gene composed of genes whose expression can be imaged with different imaging modalities. Here, we make use of a fluc reporter gene sequence to target cells via bioluminescence imaging.
FAQs
Image contrast arises when different parts of a specimen alter transmitted light to different degrees. Absorption reduces the light reaching the observer, while scattering and refraction redirect or modify it as light passes through cells, tissues, microorganisms, or other structures. These differences allow unstained or stained features to appear against the bright background.
Stains increase visual contrast when natural differences within a specimen are too subtle to distinguish clearly. In infection and immunology studies, staining can support the identification of pathogens or immune-associated features while also revealing structural patterns in cells and tissues. The resulting image remains suitable for direct visual examination using transmitted visible light.
The observable pattern depends on how the specimen interacts with transmitted light through absorption, scattering, and refraction. Cells, tissues, microorganisms, and stained preparations may therefore produce different levels or distributions of contrast. Selecting a specimen with visible structural or staining-related differences determines whether the method can show useful morphology or organization.
A basic examination directs visible light through the specimen and then uses the objective lens to form the image. The observer evaluates contrast produced by the stain and by the specimen’s optical properties against the bright background. This workflow supports visual assessment of cellular structure, tissue organization, or staining-based microbial features.
It is useful for routine examination of cell morphology, microbial growth, tissue organization, and stained features associated with pathogens or immune responses. Because the instrumentation is accessible and image formation is straightforward, laboratories can apply it to regular analysis and teaching, while also using the observations as a complement to specialized microscopy.
Researchers can assess visible differences in cellular form, the organization of tissues, microbial growth patterns, and features highlighted by staining. In infection-focused work, stained specimens may provide information relevant to pathogen identification. In immunology, images can contribute to examination of immune-associated features, although the technique may be paired with more specialized microscopy for complementary information.