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Visualizando morfologia Astrocitante usando Lúcifer iontoforese amarela

Article DOI: 10.3791/60225-v • 07:38 min • September 14th, 2019
September 14th, 2019 •
1, 1, 1, 1,2
1Department of Physiology, David Geffen School of Medicine, University of California Los Angeles, 2Department of Neurobiology, David Geffen School of Medicine, University of California Los Angeles

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Os astrócitos são células morfologicamente complexas, exemplificadas por seus múltiplos processos e territórios Bushy. Para analisar sua morfologia elaborada, apresentamos um protocolo confiável para a realização de iontoforese intracelular de Lúcifer amarelo em tecido levemente fixo.

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Neurociência edição 151 Lúcifer amarelo astrocite morfologia enchimento de corante pés finais Aquaporin-4 reconstrução 3D hipocampo
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