Journal
/
Neuroscience
/
Visualizing and Analyzing Intracellular Transport of Organelles and Other Cargos in Astrocytes
JoVE Journal
Neuroscience
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Neuroscience
Visualizing and Analyzing Intracellular Transport of Organelles and Other Cargos in Astrocytes

Visualizing and Analyzing Intracellular Transport of Organelles and Other Cargos in Astrocytes

DOI:
10.3791/60230-v

•

00:07 min

•

August 28, 2019

•

, ,
1Department of Cell Biology and Physiology,University of North Carolina at Chapel Hill

Chapters

  • 00:04Title
  • 00:31Fluorescently-Tagged Plasmid Transfection and Fluorescent Probe Late Endosome/Lysosome Labeling
  • 02:13Time-Lapse Imaging
  • 03:03Image Analysis
  • 05:00Results: Representative Quantitative Analyses of Cargo Dynamics in Astrocytes
  • 06:38Conclusion

Summary

Automatic Translation

Automatic Translation

Here we describe an in vitro live-imaging method to visualize intracellular transport of organelles and trafficking of plasma membrane proteins in murine astrocytes. This protocol also presents an image analysis methodology to determine cargo transport itineraries and kinetics.

Tags

Keywords: Intracellular TransportOrganelle MotilityAstrocytesLive ImagingFluorescent LabelingLipofectionTransfectionTime-lapse MicroscopyImage Analysis

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/60230?article_type=v

Fluorescence Recovery After Photobleaching (FRAP) of Fluorescence Tagged Proteins in Dendritic Spines of Cultured Hippocampal Neurons
check_url/60230?article_type=v

Isolation and Culture of Mouse Cortical Astrocytes
check_url/60230?article_type=v

Imaging Intracellular Ca2+ Signals in Striatal Astrocytes from Adult Mice Using Genetically-encoded Calcium Indicators
check_url/60230?article_type=v

Protocol for Three-dimensional Confocal Morphometric Analysis of Astrocytes
check_url/60230?article_type=v

Analyzing Dendritic Morphology in Columns and Layers
check_url/60230?article_type=v

Visualization and Live Imaging of Oligodendrocyte Organelles in Organotypic Brain Slices Using Adeno-associated Virus and Confocal Microscopy
check_url/60230?article_type=v

Analyzing the Size, Shape, and Directionality of Networks of Coupled Astrocytes
check_url/60230?article_type=v

Analyzing Mitochondrial Transport and Morphology in Human Induced Pluripotent Stem Cell-Derived Neurons in Hereditary Spastic Paraplegia
check_url/60230?article_type=v

Axonal Transport of Organelles in Motor Neuron Cultures using Microfluidic Chambers System
check_url/60230?article_type=v

In Vitro Wedge Slice Preparation for Mimicking In Vivo Neuronal Circuit Connectivity

Read Article