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JoVE Journal Neuroscience
Visualizing and Analyzing Intracellular Transport of Organelles and Other Cargos in Astrocytes
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Visualizing and Analyzing Intracellular Transport of Organelles and Other Cargos in Astrocytes

Article DOI: 10.3791/60230-v • 07:19 min • August 28th, 2019
August 28th, 2019 •
*1, *1, 1
1Department of Cell Biology and Physiology, University of North Carolina at Chapel Hill
* These authors contributed equally

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Summary
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Here we describe an in vitro live-imaging method to visualize intracellular transport of organelles and trafficking of plasma membrane proteins in murine astrocytes. This protocol also presents an image analysis methodology to determine cargo transport itineraries and kinetics.

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Visualizing Analyzing Intracellular Transport Organelles Cargos Astrocytes Motility Localization Live Imaging Technique Transfection Lipofection Reagent DNA Lipofection Enhancer Reagent Astrocyte Culture Medium Lysosomal Labeling Probe
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