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Neuroscience

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Visualizing and Analyzing Intracellular Transport of Organelles and Other Cargos in Astrocytes
 

Visualizing and Analyzing Intracellular Transport of Organelles and Other Cargos in Astrocytes

Article doi: 10.3791/60230
August 28th, 2019

Summary August 28th, 2019

Here we describe an in vitro live-imaging method to visualize intracellular transport of organelles and trafficking of plasma membrane proteins in murine astrocytes. This protocol also presents an image analysis methodology to determine cargo transport itineraries and kinetics.

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