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Bulk Droplet Vitrification for Primary Hepatocyte Preservation
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Bioengineering
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JoVE Journal Bioengineering
Bulk Droplet Vitrification for Primary Hepatocyte Preservation

Bulk Droplet Vitrification for Primary Hepatocyte Preservation

DOI:
10.3791/60250-v

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11:07 min

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October 25, 2019

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, , , , , , , ,
1Center for Engineering in Medicine,Harvard Medical School, Massachusetts General Hospital, 2Shriners Hospitals for Children, Boston, 3Department of Surgery,University of Amsterdam

Chapters

  • 00:04Title
  • 01:01Vitrification Solutions and Apparatus Preparation
  • 03:47Hepatocyte Pre-incubation with Cryoprotective Agents (CPAs)
  • 04:59Bulk Droplet Vitrification
  • 05:51Cryogenic Storage
  • 07:00Rewarming of the Vitrified Hepatocyte Droplets
  • 09:05Results: Comparison of Bulk Droplet Vitrification to Classic Cryopreservation
  • 10:21Conclusion

Summary

Automatic Translation

Automatic Translation

This manuscript describes an ice-free cryopreservation method for large quantities of rat hepatocytes whereby primary cells are pre-incubated with cryoprotective agents at a low concentration and vitrified in large droplets.

Tags

Keywords: Bulk Droplet VitrificationPrimary Hepatocyte PreservationCryoprotective AgentsOsmotic DehydrationCell CryopreservationHepatocyte TransplantationBio-artificial LiverBlood ProductsStem Cells

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