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Enrichment of Pachytene Spermatocytes and Spermatids from Mouse Testes Using Standard Laboratory Equipment
JoVE Journal
Developmental Biology
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JoVE Journal Developmental Biology
Enrichment of Pachytene Spermatocytes and Spermatids from Mouse Testes Using Standard Laboratory Equipment

Enrichment of Pachytene Spermatocytes and Spermatids from Mouse Testes Using Standard Laboratory Equipment

DOI:
10.3791/60271-v

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10:22 min

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September 17, 2019

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, , , ,
1Institute of Biomedicine,University of Turku

Chapters

  • 00:04Title
  • 00:58Animal Dissection and Preparation of Germ Cell Suspension
  • 04:50Separation of Germ Cells Through the Discontinuous BSA Gradient
  • 06:02Collection of Enriched Germ Cell Fractions
  • 06:52Analysis of Cell Fractions
  • 08:06Results: MDR Cell Enrichment
  • 09:29Conclusion

Summary

Automatic Translation

Automatic Translation

Presented here is a protocol for enriching pachytene spermatocytes, round spermatids, and elongating spermatids from adult mouse testes using a discontinuous bovine serum albumin density gradient with standard laboratory equipment.

Tags

Keywords: Pachytene SpermatocytesSpermatidsMouse TestesMDR MethodStandard Laboratory EquipmentSpermatogenesisMale FertilityPaternal Epigenetic InheritanceTestis DissectionCollagenaseTrypsinCell Separation

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