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In Vivo Optical Calcium Imaging of Learning-Induced Synaptic Plasticity in Drosophila melanogaster
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Chapters
- 00:04Title
- 00:26In Vivo Calcium Imaging Preparation
- 02:44In Vivo Calcium Imaging
- 03:39Olfactory Conditioning and Odor-Evoked Calcium Transients Visualization
- 05:07Results: Representative Postsynaptically-Localized Calcium and Learning-Induced Postsynaptic Plasticity Visualization
- 05:56Conclusion
Here we present a protocol with which pre- and/or postsynaptic calcium can be visualized in the context of Drosophila learning and memory. In vivo calcium imaging using synaptically localized calcium sensors is combined with a classical olfactory conditioning paradigm such that the synaptic plasticity underlying this type of associative learning may be determined.
