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Experimental Approach to Examine Leptin Signaling in the Carotid Bodies and its Effects on Control of Breathing

Article DOI: 10.3791/60298-v • 05:45 min • October 25th, 2019
October 25th, 2019 •
1, 1, 2, 1
1Department of Medicine, Johns Hopkins University, 2Instituto de Biomedicina de Sevilla (IBiS), Centro de Investigación Biomédica en Red de Enfermedades Respiratorias (CIBERES), Hospital Universitario Virgen del Rocío/Universidad de Sevilla

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Summary
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Our study focuses on the effects of leptin signaling in carotid body (CB) on the hypoxic ventilatory response (HVR). We performed 'loss of function' experiments measuring the effect of leptin on HVR after CB denervation and 'gain of function' experiments measuring HVR after overexpression of the leptin receptor in CB.

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Experimental Approach Leptin Signaling Carotid Bodies Control Of Breathing Respiratory Effects Peripheral Chemoreflex Hypoxia Carotid Body Function Surgical Denervation Chemical Denervation Adenovirus Transfection Whole Body Plasthemagrophy Chamber Temperature Measurement Humidity Measurement Body Weight Measurement Oximeter Collar Normoxia Mixed Gas Flow Acute Hypoxia Respiratory Signals Peripheral Oxygen Saturation
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