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JoVE Journal
Genetics

This content is Open Access.

Dissection of Drosophila melanogaster Flight Muscles for Omics Approaches
 

Dissection of Drosophila melanogaster Flight Muscles for Omics Approaches

Article doi: 10.3791/60309
October 17th, 2019

Summary October 17th, 2019

Drosophila flight muscle is a powerful model to study transcriptional regulation, alternative splicing, metabolism, and mechanobiology. We present a protocol for dissection of fluorescent-labeled flight muscle from live pupae to generate highly enriched samples ideal for proteomics and deep-sequencing. These samples can offer important mechanistic insights into diverse aspects of muscle development.

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