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Dissection of Drosophila melanogaster Flight Muscles for Omics Approaches
JoVE Journal
Genetics
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JoVE Journal Genetics
Dissection of Drosophila melanogaster Flight Muscles for Omics Approaches

Dissection of Drosophila melanogaster Flight Muscles for Omics Approaches

DOI:
10.3791/60309-v

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08:33 min

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October 17, 2019

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, , ,
1Biomedical Center, Department of Physiological Chemistry,Ludwig-Maximilians-University Munich, 2Center for Integrated Protein Science Munich (CIPSM), Department of Chemistry,Ludwig-Maximilians-University Munich

Chapters

  • 00:04Title
  • 00:51Pupae Staging
  • 01:28Indirect Flight Muscle (IFM) Dissection Before 48 Hours After Puparium Formation (APF)
  • 03:20IFM Dissection After 48 Hours APF
  • 06:04Results: Representative Application of IFM Dissections to Investigate Bruno1 Function in Alternative Splicing
  • 07:40Conclusion

Summary

Automatic Translation

Automatic Translation

Drosophila flight muscle is a powerful model to study transcriptional regulation, alternative splicing, metabolism, and mechanobiology. We present a protocol for dissection of fluorescent-labeled flight muscle from live pupae to generate highly enriched samples ideal for proteomics and deep-sequencing. These samples can offer important mechanistic insights into diverse aspects of muscle development.

Tags

Keywords: Drosophila MelanogasterFlight MusclesDissectionOmics ApproachesGFP-labeledIndirect Flight MusclePupae DevelopmentGene ExpressionProtein ExpressionRT-PCRWestern BlotStagingSexingFluorescent MicroscopyForcepsPBS

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