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JoVE Journal
Immunology and Infection

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Pneumococcus Infection of Primary Human Endothelial Cells in Constant Flow
 

Pneumococcus Infection of Primary Human Endothelial Cells in Constant Flow

Article doi: 10.3791/60323
October 31st, 2019

Summary October 31st, 2019

This study describes the microscopic monitoring of pneumococcus adherence to von Willebrand factor strings produced on the surface of differentiated human primary endothelial cells under shear stress in defined flow conditions. This protocol can be extended to detailed visualization of specific cell structures and quantification of bacteria by applying differential immunostaining procedures.

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