A Semi-high-throughput Imaging Method and Data Visualization Toolkit to Analyze <em>C. elegans</em> Embryonic Development

Renat  N. Khaliullin; Jeffrey  M. Hendel; Adina Gerson-Gurwitz; Shaohe Wang; Stacy  D. Ochoa; Zhiling Zhao; Arshad Desai; Karen Oegema; Rebecca  A. Green

doi:10.3791/60362

Journal

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Developmental Biology

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A Semi-high-throughput Imaging Method and Data Visualization Toolkit to Analyze C. elegans Embryonic Development

A Semi-high-throughput Imaging Method and Data Visualization Toolkit to Analyze <em>C. elegans</em> Embryonic Development

JoVE Journal
Developmental Biology

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JoVE Journal Developmental Biology

A Semi-high-throughput Imaging Method and Data Visualization Toolkit to Analyze C. elegans Embryonic Development

A Semi-high-throughput Imaging Method and Data Visualization Toolkit to Analyze C. elegans Embryonic Development

6,673 Views

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06:49 min

•

October 29, 2019

DOI:

10.3791/60362-v

DOI:

10.3791/60362-v

•

06:49 min

October 29, 2019

•

6659 Views

Renat N. Khaliullin^1,2,3, Jeffrey M. Hendel^1,2, Adina Gerson-Gurwitz^1,2, Shaohe Wang^1,4,5, Stacy D. Ochoa^1,6, Zhiling Zhao^1,7, Arshad Desai^1,2, Karen Oegema^1,2, Rebecca A. Green^1,2

¹Ludwig Institute for Cancer Research, San Diego ²Department of Cellular and Molecular Medicine,University of California, San Diego ³Recursion Pharmaceuticals ⁴Biomedical Sciences Graduate Program,University of California, San Diego ⁵Cell Biology Section, National Institute of Dental and Craniofacial Research,National Institutes of Health ⁶Department of Biology,San Diego State University ⁷Developmental and Stem Cell Biology Graduate Program,University of California, San Francisco

Summary

This work describes a semi-high-throughput protocol that allows simultaneous 3D time-lapse imaging of embryogenesis in 80–100 C. elegans embryos in a single overnight run. Additionally, image processing and visualization tools are included to streamline data analysis. The combination of these methods with custom reporter strains enables detailed monitoring of embryogenesis.