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Journal / Cancer Research / Etablierung und Analyse von dreidimensionalen…
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JoVE Journal Cancer Research
Etablierung und Analyse von dreidimensionalen (3D) Organoiden, abgeleitet von Patienten prostatakrebs Knochenmetastasen Proben und ihre Xenografts
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Etablierung und Analyse von dreidimensionalen (3D) Organoiden, abgeleitet von Patienten prostatakrebs Knochenmetastasen Proben und ihre Xenografts

Article DOI: 10.3791/60367-v • 07:21 min • February 3rd, 2020
February 3rd, 2020 •
1,2, 1,2, 1,2, 1,2, 1,2, 2,3, 4, 2,5, 1,2, 1,2
1Department of Urology, University of California, San Diego, 2Moores Cancer Center, University of California, San Diego, 3Department of Medicine, University of California, San Diego, 4Department of Radiation Oncology, University of California, Los Angeles, 5Department of Orthopedic Surgery, University of California, San Diego

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Dreidimensionale Kulturen von Patienten-BMPC-Proben und Xenografts von Knochenmetastasierung Prostatakrebs erhalten die funktionelle Heterogenität ihrer ursprünglichen Tumoren, was zu Zysten, Sphäroiden und komplexen, tumorartigen Organoiden führt. Dieses Manuskript bietet eine Optimierungsstrategie und ein Protokoll für die 3D-Kultur heterogener Patientenproben und deren Analyse mit IFC.

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In diesem Monat in JoVE Ausgabe 156 Patient Derived Organoide (g.U.) Spin Down Methods Bone Metastatic Prostate Cancer (BMPC) In Vivo Stitch Imaging Immunofluoreszenz Zytochemie (IFC)
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