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Developmental Biology
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多细胞人类原发性子宫内膜器官的生成
JoVE Journal
Developmental Biology
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JoVE Journal
Developmental Biology
Generation of Multicellular Human Primary Endometrial Organoids
Please note that all translations are automatically generated.
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多细胞人类原发性子宫内膜器官的生成
DOI:
10.3791/60384-v
•
09:20 min
•
October 04, 2019
•
Alina R. Murphy*
1
,
Teerawat Wiwatpanit*
2
,
Zhenxiao Lu
,
Batzaya Davaadelger
,
J. Julie Kim
1
Department of Obstetrics and Gynecology
,
Northwestern University
,
2
National Center for Genetic Engineering and Biotechnology (BIOTEC)
,
National Science and Technology Development Agency (NSTDA)
Chapters
00:04
Title
01:39
Preparation of Agarose Molds and Generation of Endometrial Organoids
04:58
Harvesting Endometrial Organoids for Experiments
06:40
Results: Analysis of Generated Multicellular Human Primary Endometrial Organoids
08:10
Conclusion
Summary
Automatic Translation
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Automatic Translation
提出了一种产生人类原发子宫内膜器官的方案,该细胞由上皮细胞和基质细胞组成,并保留原生子宫内膜组织的特性。该协议描述了从子宫组织采集到子宫内膜器官组织处理的方法。
Tags
Endometrium
Organoids
3D Structure
Epithelial Cells
Stromal Cells
Hormone Imbalance
Cancer
Paracrine Actions
Cell Isolation
Enzyme Solution
Cell Strainer
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