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Native polyakrylamid gel elektrofores immunoblot analys av endogena IRF5 Dimerization
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Native Polyacrylamide Gel Electrophoresis Immunoblot Analysis of Endogenous IRF5 Dimerization
DOI:

08:57 min

October 06, 2019

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Chapters

  • 00:04Title
  • 01:00Stimulation of CAL-1 Cells
  • 02:23Extraction of Cellular Proteins
  • 03:39Analysis of IRF5 Dimerization by Native PAGE
  • 04:55Immunoblot Analysis of IRF5
  • 06:10Results: IRF5 Dimerization
  • 07:21Conclusion

Summary

Automatic Translation

En Native Western blot metod för att analysera endogena interferon reglerande faktor 5 Dimerization i Cal-1 dess dendritiska cellinjer beskrivs. Detta protokoll kan även tillämpas på andra cellinjer.

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