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Biochemistry

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JoVE Journal Biochemistry
Profiling Ubiquitin and Ubiquitin-like Dependent Post-translational Modifications and Identification of Significant Alterations
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Profiling Ubiquitin and Ubiquitin-like Dependent Post-translational Modifications and Identification of Significant Alterations

Article DOI: 10.3791/60402-v • 10:26 min • November 7th, 2019
November 7th, 2019 •
1, 1, 1, 1, 1, 1, 1, 1
1Aix-Marseille Univ, INSERM, CNRS, Institut Paoli-Calmettes, CRCM

Chapters

Summary
Automatic Translation

This protocol aims at establishing ubiquitin (Ub) and ubiquitin-likes (Ubls) specific proteomes in order to identify alterations of these kind of post-translational modifications (PTMs), associated with a specific condition such as a treatment or a phenotype.

Tags

Ubiquitin Ubiquitin-like Post-translational Modifications Lentiviruses Stable Cell Line Tags Purification Biological Functions Disease Prognostic Diagnosis Molecular Targets Conserved Protein Eukaryotic Cells Method Lentiviral System Incubation Time Eluate Nickel Beads Flag Beads Critical Steps Purification Process
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