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In Vitro Assessment of Cardiac Function Using Skinned Cardiomyocytes
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JoVE Journal Medicine
In Vitro Assessment of Cardiac Function Using Skinned Cardiomyocytes

In Vitro Assessment of Cardiac Function Using Skinned Cardiomyocytes

DOI:
10.3791/60427-v

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08:19 min

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June 22, 2020

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, , , , , ,
1Unidade de Investigação Cardiovascular, Departamento de Cirurgia e Fisiologia, Faculdade de Medicina,Universidade do Porto, 2Department of Physiology,Kilimanjaro Christian Medical University College

Chapters

  • 00:04Introduction
  • 01:00Skinned Cardiomyocyte Extraction and Permeabilization
  • 02:46Skinned Cardiomyocyte Selection and Gluing
  • 04:03Active, Passive and Calcium (Ca2+) Sensitivity Force Measurements
  • 05:58Results: Representative Skinned Cardiomyocyte Assessment
  • 07:36Conclusion

Summary

Automatic Translation

Automatic Translation

This protocol aims to describe step-by-step the technique of extraction and assessment of cardiac function using skinned cardiomyocytes. This methodology allows measurement and acutemodulation of myofilament function using small frozen biopsies that can be collected from different cardiac locations, from mice to men.

Tags

Keywords: In Vitro Cardiac FunctionSkinned CardiomyocytesMyofilament FunctionTherapeutic InterventionsCardiomyocyte ExtractionForce TransducerTritonRelax ISO SolutionCell SuspensionRod-shaped CardiomyocyteStriation Pattern

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