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Immunology and Infection
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Culture Methods to Determine the Limit of Detection and Survival in Transport Media of Campylobacter Jejuni in Human Fecal Specimens
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Culture Methods to Determine the Limit of Detection and Survival in Transport Media of Campylobacter Jejuni in Human Fecal Specimens

Culture Methods to Determine the Limit of Detection and Survival in Transport Media of Campylobacter Jejuni in Human Fecal Specimens

DOI:
10.3791/60457-v

•

08:23 min

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March 10, 2020

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, ,
1TECHLAB, Inc.

Chapters

  • 00:04Introduction
  • 01:04Campylobacter Enumeration in Contrived Human Fecal Specimens
  • 03:42Contrived Clinical Fecal Specimen Preparation and Enumeration
  • 05:21Determination of Campylobacter Viability in Transport Medium
  • 06:17Results: Representative Typical Numbers of Colonies on Plates of Spiked Fecal Samples
  • 07:10Conclusion

Summary

Automatic Translation

Automatic Translation

Although stool culture for Campylobacter is imprecise, it is still considered the gold standard for identification. Methods to determine the limit of detection and survival in transport media of C. jejuni in human stool are described and compared with a new immunoassay with better accuracy.

Tags

Keywords: Campylobacter JejuniCultureDetectionSurvivalTransport MediaFecal SpecimensLimit Of DetectionAnaerobicMicroaerobicOptical DensityDilution SeriesPlate Counting

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