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使用Tg(Vtg1:mcherry)斑马鱼胚胎测试内分泌干扰化合物的雌激素效应
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Using Tg(Vtg1:mcherry) Zebrafish Embryos to Test the Estrogenic Effects of Endocrine Disrupting Compounds
Please note that all translations are automatically generated.
Click here for the English version.
使用Tg(Vtg1:mcherry)斑马鱼胚胎测试内分泌干扰化合物的雌激素效应
DOI:
10.3791/60462-v
•
12:02 min
•
August 08, 2020
•
Zsolt Csenki
,
Ákos Horváth
,
Illés Bock
,
Edina Garai
,
Flóra Kerekes
,
Erna Vásárhelyi
,
Balázs Kovács
,
Béla Urbányi
,
Ferenc Mueller
,
Katalin Bakos
1
Department of Aquaculture, Institute of Aquaculture and Environmental Safety, Faculty of Agricultural and Environmental Sciences
,
Szent István University
,
2
Institute of Cancer and Genomic Sciences, College of Medical and Dental Sciences
,
University of Birmingham
Chapters
00:00
Introduction
02:52
Embryon Harvest and Treatment
05:05
Larvae Preparation fo Photography
06:05
Microscopy
07:02
Determining Integrated Density
08:14
Representative Results
10:20
Conclusions
Summary
Automatic Translation
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Automatic Translation
目前这里是使用斑马鱼胚胎
Tg(vtg1:mCherry)
来检测雌激素效应的详细协议。该协议涵盖鱼类的繁殖和胚胎的治疗,并强调内分泌干扰化合物(EDC)引起的荧光信号的检测、文献和评估。
Tags
Tg(Vtg1:mcherry) Zebrafish
Endocrine Disrupting Compounds
Estrogenic Effects
Biomarker
Vitellogenin
In Vivo Testing
Environmental Samples
Animal Welfare
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