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Determination of Immune Cell Identity and Purity Using Epigenetic-Based Quantitative PCR
This content is Free Access.
Chapters
- 00:05Introduction
- 00:41Sample Preparation
- 01:41Bisulfate Conversion
- 02:20CD8+ T Cell and Regulatory T Cell (Treg) Assays
- 05:28T Helper Cell 17 (Th17) Assay
- 06:37Results: Representative Flow and Methylation Assay Data Comparisons
- 07:30Conclusion
Here we describe a robust method of determining immune cell identity and purity through epigenetic signatures detected using quantitative PCR (qPCR). DNA demethylation at a specific locus serves as a unique identifier for a particular cell type and allows for identification of CD8+, regulatory, or Th17 T cells.
