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JoVE Journal
Immunology and Infection

This content is Open Access.

Determination of Immune Cell Identity and Purity Using Epigenetic-Based Quantitative PCR
 

Determination of Immune Cell Identity and Purity Using Epigenetic-Based Quantitative PCR

Article doi: 10.3791/60465
February 19th, 2020

Summary February 19th, 2020

Here we describe a robust method of determining immune cell identity and purity through epigenetic signatures detected using quantitative PCR (qPCR). DNA demethylation at a specific locus serves as a unique identifier for a particular cell type and allows for identification of CD8+, regulatory, or Th17 T cells.

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