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通过清除心脏三维成像捕获目标细胞群的心脏损伤反应
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Developmental Biology
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JoVE Journal
Developmental Biology
Capturing the Cardiac Injury Response of Targeted Cell Populations via Cleared Heart Three-Dimensional Imaging
Please note that all translations are automatically generated.
Click here for the English version.
通过清除心脏三维成像捕获目标细胞群的心脏损伤反应
DOI:
10.3791/60482-v
•
08:14 min
•
March 17, 2020
•
Rebecca J. Salamon
,
Ziheng Zhang
,
Ahmed I. Mahmoud
1
Department of Cell and Regenerative Biology
,
University of Wisconsin-Madison School of Medicine and Public Health
Chapters
00:05
Introduction
00:52
Left Anterior Descending Artery (LAD) Ligation-Induced Coronary Artery Occlusion (Myocardial Infarction)
02:58
Passive Clear Lipid-Exchanged Acrylamide-Hybridized Rigid Imaging Compatible Tissue Hydrogel (CLARITY)
05:53
Single-Photon Confocal Microscopy Cleared Mouse Heart Imaging
06:28
Results: Representative Neonatal Mouse Visualization
07:46
Conclusion
Summary
Automatic Translation
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Automatic Translation
伤愈后心肌细胞增殖是一个动态过程,需要来自非肌细胞群的细胞外线索交响。利用谱系跟踪、被动CLARITY和三维全贴装共聚焦显微镜技术,可以分析各种细胞类型对心脏修复和再生的影响。
Tags
Cardiac Injury
Cell Populations
Three-dimensional Imaging
Heart Regeneration
LAD Occlusion
Myocardial Infarction
Fluorescent Staining
Microscopy
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