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Electrowetting-based Digital Microfluidics Platform for Automated Enzyme-linked Immunosorbent Assay
JoVE Journal
Bioengineering
This content is Free Access.
JoVE Journal Bioengineering
Electrowetting-based Digital Microfluidics Platform for Automated Enzyme-linked Immunosorbent Assay
DOI:

08:22 min

February 23, 2020

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Chapters

  • 00:04Title
  • 01:12Loading and Operation of the EWOD Chip on the DMF Platform
  • 04:10Operating in Visual Mode
  • 05:03Removing Liquid Waste and Cleaning the Chip
  • 05:48Results: Optimization of Immunoassays on the EWOD Chip
  • 07:24Conclusion

Summary

Automatic Translation

Electrowetting-based digital microfluidic is a technique that utilizes a voltage-driven change in the apparent contact angle of a microliter-volume droplet to facilitate its manipulation. Combining this with functionalized magnetic beads enables the integration of multiple laboratory unit operations for sample preparation and identification of pathogens using Enzyme-linked Immunosorbent Assay (ELISA).

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