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Chapters
- 00:04Title
- 01:18Serum Collection: Survival Procedure
- 02:44CerebroSpinal Fluid (CSF) Collection: Survival Procedure
- 05:05Serum and CSF Collection: Non-Survival Procedure
- 06:07Protein Analysis
- 07:02Results: Representative Blood-Brain Barrier Function and Intrathecal IgG Synthesis in Relapsing Experimental Autoimmune Encephalomyelitis (R-EAE) and Theiler’s Murine Encephalomyelitis Virus-Induced Demyelinating Disease (TMEV-IDD)
- 07:53Conclusion
Elevated spinal fluid protein levels can either be the result of diffusion of plasma protein across an altered blood-brain barrier or intrathecal synthesis. An optimized testing protocol is presented in this article that helps to discriminate both cases and provides quantitative measurements of intrathecally synthesized proteins.
