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Developmental Biology

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JoVE Journal Developmental Biology
Whole-Mount In Situ Hybridization in Zebrafish Embryos and Tube Formation Assay in iPSC-ECs to Study the Role of Endoglin in Vascular Development
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Whole-Mount In Situ Hybridization in Zebrafish Embryos and Tube Formation Assay in iPSC-ECs to Study the Role of Endoglin in Vascular Development

Article DOI: 10.3791/60498-v • 08:27 min • May 28th, 2020
May 28th, 2020 •
*1, *2, 2, 2, 1, 3, 1,2
1Department of Physiology and Department of Cardiology of the Second Affiliated Hospital, Zhejiang University School of Medicine, 2Department of Cell Biology, State Key Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences and Peking Union Medical College, 3Wuxi Lung Transplant Center, Wuxi People's Hospital affiliated to Nanjing Medical University
* These authors contributed equally

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Summary
Automatic Translation

Presented here is a protocol for whole-mount in situ RNA hybridization analysis in zebrafish and tube formation assay in patient-derived induced pluripotent stem cell-derived endothelial cells to study the role of endoglin in vascular formation.

Tags

Whole-mount In Situ Hybridization WISH Zebrafish Embryos Tube Formation Assay IPSC-ECs Endoglin Vascular Development U-slide Plates Mouse Embryos Drosophila Embryos Stem Cells Androgen Cells Mutation Correction Yong Wang Research Assistant FemtoJet System Microinjection Morpholinos MRNA Eggs Zygotes Dechorionated 4% PFA Solution
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