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Developmental Biology
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用于基因表达分析的小鼠胚胎软骨和骨骼的激光捕获微分
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Developmental Biology
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JoVE Journal
Developmental Biology
Laser Capture Microdissection of Mouse Embryonic Cartilage and Bone for Gene Expression Analysis
Please note that all translations are automatically generated.
Click here for the English version.
用于基因表达分析的小鼠胚胎软骨和骨骼的激光捕获微分
DOI:
10.3791/60503-v
•
09:20 min
•
December 18, 2019
•
Meng Wu
,
Divya Kriti
,
Harm van Bakel
2
,
Ethylin Wang Jabs
,
Greg Holmes
1
Department of Genetics and Genomic Sciences
,
Icahn School of Medicine at Mount Sinai
,
2
Icahn Institute for Data Science and Genomic Technology
,
Icahn School of Medicine at Mount Sinai
Chapters
00:04
Title
01:04
Preparation of Fresh Frozen Specimen
01:28
Cryosectioning for Laser Capture Microdissection
02:45
Sample Preparation for LCM
04:14
Laser Capture Microdissection
06:25
Lysis of Microdissected Tissues and RNA Isolation
06:54
Results: Analysis of Laser Capture Microdissected Tissue
08:49
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
该协议描述了激光捕获微解剖,用于从小鼠胚胎的新鲜冷冻部分分离软骨和骨骼。软骨和骨骼可以通过紫罗兰染色快速可视化,并精确收集,产生高质量的RNA进行转录分析。
Tags
Laser Capture Microdissection
Mouse Embryonic Cartilage
Mouse Embryonic Bone
Gene Expression Analysis
Cresyl Violet Staining
RNA Integrity
Cryosectioning
Optimal Cutting Temperature Compound
PEN Membrane Slides
Ethanol
Xylene
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