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Study of Short Peptide Adsorption on Solution Dispersed Inorganic Nanoparticles Using Depletion Method
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JoVE Journal Chemistry
Study of Short Peptide Adsorption on Solution Dispersed Inorganic Nanoparticles Using Depletion Method

Study of Short Peptide Adsorption on Solution Dispersed Inorganic Nanoparticles Using Depletion Method

DOI:
10.3791/60526-v

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09:43 min

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April 11, 2020

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, , , ,
1Nanotechnology Education and Research Center,South Ural State University, 2Laboratory of Computational Modelling of Drugs,South Ural State University

Chapters

  • 00:00Introduction
  • 00:58Preparation of 16 mM Dipeptide Stock Solution
  • 02:18Preparation of Peptide Dilutions
  • 03:25Preparation of Titania Sole
  • 04:26Mixing and Thermostating
  • 05:32Filtration of Thermostated Samples
  • 06:10Preparation of Derivatization Solutions
  • 06:58Derivatization
  • 07:37HPLC Analysis
  • 07:58Results
  • 09:05Conclusion

Summary

Automatic Translation

Automatic Translation

The first step in comprehending biomolecule-inorganic solid phase interaction is revealing fundamental physicochemical constants that may be evaluated by establishing adsorption isotherms. Adsorption from the liquid phase is restricted by kinetics, surface capacity, pH, and competitive adsorption, which all should be cautiously considered before setting an adsorption experiment.

Tags

Keywords: Peptide AdsorptionInorganic NanoparticlesDepletion MethodAdsorption IsothermsDipeptide AdsorptionTitanium DioxidePH AdjustmentMES BufferSolution PreparationConcentration Dilution

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