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JoVE Journal Genetics
Use of the Pyrimidine Analog, 5-Iodo-2′-Deoxyuridine (IdU) with Cell Cycle Markers to Establish Cell Cycle Phases in a Mass Cytometry Platform
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Use of the Pyrimidine Analog, 5-Iodo-2′-Deoxyuridine (IdU) with Cell Cycle Markers to Establish Cell Cycle Phases in a Mass Cytometry Platform

Article DOI: 10.3791/60556-v • 08:37 min • October 22nd, 2021
October 22nd, 2021 •
1, 1
1Ohio State Comprehensive Cancer Center, Division of Hematology, The Ohio State University

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This protocol adapts cell cycle measurements for use in a mass cytometry platform. With the multi-parameter capabilities of mass cytometry, direct measurement of iodine incorporation allows identification of cells in S-phase while intracellular cycling markers enable characterization of each cell cycle state in a range of experimental conditions.

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Pyrimidine Analog 5-Iodo-2'-Deoxyuridine (IdU) Cell Cycle Markers Mass Cytometry Platform Single Cell Level Research Clinical Studies Simplicity Acute Myeloid Leukemia Stem Cells Progenitor Cells Clinical Outcome Rare Cancer Cells Immune Cell Subsets Growth Conditions IdU Stock Solution Growth Media Biosafety Hood Centrifugation
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