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JoVE Journal Genetics
Anvendelse af pyrimidinanalogen, 5-Iodo-2′-deoxyuridin (IdU) med cellecyklusmarkører til at etablere cellecyklusfaser i en massecytometriplatform
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Anvendelse af pyrimidinanalogen, 5-Iodo-2′-deoxyuridin (IdU) med cellecyklusmarkører til at etablere cellecyklusfaser i en massecytometriplatform

Article DOI: 10.3791/60556-v • 08:37 min • October 22nd, 2021
October 22nd, 2021 •
1, 1
1Ohio State Comprehensive Cancer Center, Division of Hematology, The Ohio State University

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Denne protokol tilpasser cellecyklusmålinger til brug i en massecytometriplatform. Med multiparameterfunktionerne i massecytometri muliggør direkte måling af jodinkorporering identifikation af celler i S-fase, mens intracellulære cykelmarkører muliggør karakterisering af hver cellecyklustilstand under en række eksperimentelle betingelser.

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Tilbagetrækning udgave 176 massecytometri cellecyklus iodo-deoxyuridin bestråling DNA-skaderespons cellelinje immunceller
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