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Visualizing the Developing Brain in Living Zebrafish using Brainbow and Time-lapse Confocal Imaging
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Chapters
- 00:05Introduction
- 00:53Microinjection and Heat Shock of Zebrafish Embryos
- 02:06Screening and Mounting Embryos
- 03:59Time-lapse Confocal Imaging of the Developing Zebrafish Hindbrain
- 05:17Results: Brainbow Labels Clonally Related Clusters of Dividing Cells in the Developing Zebrafish Hindbrain
- 06:55Conclusion
In vivo imaging is a powerful tool that can be used to investigate the cellular mechanisms underlying nervous system development. Here we describe a technique for using time-lapse confocal microscopy to visualize large numbers of multicolor Brainbow-labeled cells in real time within the developing zebrafish nervous system.
