Cell Division Tracking
Cell Division Tracking - Related Videos
22:49
Optimized Staining and Proliferation Modeling Methods for Cell Division Monitoring using Cell Tracking Dyes
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Cited by 45 •
2012
Successful use of cell tracking dyes to monitor immune cell function and proliferation involves several critical steps. We describe methods for: 1) obtaining bright, uniform, reproducible label-ing with membrane dyes; 2) selecting fluorochromes and data acquisition conditions; and 3) choosing a model to quantify cell proliferation based on dye dilution.
10:03
An Introduction to Cell Division
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2023
Cell division is the process by which a parent cell divides and gives rise to two or more daughter cells. It is a means of reproduction for single-cell organisms. In multicellular organisms, cell division contributes to growth, development, repair, and the generation of reproductive cells (sperms and eggs). Cell division is a tightly regulated process, and aberrant cell division can cause diseases, notably cancer. JoVE's Introduction to Cell Division will cover a brief history of the landmark...
05:44
Cell Division - Concepts
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2019
Cell division is fundamental to all living organisms and required for growth and development. As an essential means of reproduction for all living things, cell division allows organisms to transfer their genetic material to their offspring. For a unicellular organism, cellular division generates a completely new organism. For multicellular organisms, cellular division produces new cells for the general development of the organism, as well as produces healthy cells to replace damaged cells from...
06:20
Cell Division - Student Protocol
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2019
Observing the Cell Cycle in a Root Tip ExpandHypotheses: The experimental hypothesis is that in root tips slices that have been treated with nocodazole, a chemical that interferes with microtubular polymerization, all of the cells will be arrested at the same stage of the cell cycle and that in untreated onion tip slices all of the different stages of the cell cycle will be visualized. The null hypothesis is that there will be no differences in mitosis between the two groups and that different...
00:53
Cell Division - Instructor Prep
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2019
Preparation of Onion Root Tips and Solutions ExpandFor the cell cycle experiment using root tips, first, leave an onion suspended over a beaker of water to grow roots for several days. Then, clean the onion roots of any dirt or debris. Next, in a 1.5 mL tube, dissolve 10 mcg of nocodazole per 1 mL of dimethyl sulfoxide solution, making 1 tube per student. Finally, set out all of the other tools, materials, and reagents from the list of materials onto each student's lab bench.
FAQs
These tools make cellular events visible during observation, allowing researchers to associate a signal with a mitotic event and follow labeled cells through later divisions. Fluorescent labels provide an imaging readout, whereas genetically encoded reporters serve as cellular markers. Combined with time-lapse microscopy, they help connect division timing to the identity and location of cells in a lineage.
Recording when divisions occur adds a temporal dimension to measurements of proliferation. Researchers can determine whether cells divide at similar or different times and relate that timing to cell-cycle regulation, developmental signals, or tissue organization. These observations help explain how individual cellular behaviors contribute to the growth and changing structure of a cell population.
Following daughter cells across successive generations preserves the history of how a population develops from individual divisions. This makes it possible to connect an earlier mitotic event with later cellular behavior, rather than treating every cell as an unrelated observation. Such lineage-based analysis supports studies of cellular fate, stem cells, development, and tissue regeneration.
A typical workflow begins by marking cells with fluorescent labels or genetically encoded reporters, then observing them with time-lapse microscopy. Researchers record the locations and timing of mitotic events, identify daughter cells, and trace their progression through subsequent generations. The resulting observations can be used to measure division timing and relate individual histories to population-level growth.
Cell division tracking can examine how cell-cycle regulation, tissue organization, and developmental signals influence proliferation. It is also relevant to stem cell behavior, cancer biology, and tissue regeneration, where the history of individual divisions may clarify how cells contribute to a larger tissue or population. This connects cellular dynamics with biological processes occurring across multiple organizational levels.
The approach allows researchers to examine drug responses through individual cellular behaviors rather than population growth alone. By recording mitotic events, division timing, and daughter-cell lineages, investigators can relate changes in cellular histories to changes in overall population growth and fate. This provides a way to study how responses vary across cells within the same biological system.