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Journal
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Biochemistry
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使用微尺度热泳测量蛋白质-脂质相互作用
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Biochemistry
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JoVE Journal
Biochemistry
Use of Microscale Thermophoresis to Measure Protein-Lipid Interactions
Please note that all translations are automatically generated.
Click here for the English version.
使用微尺度热泳测量蛋白质-脂质相互作用
DOI:
10.3791/60607-v
•
04:45 min
•
February 10, 2022
•
Robert P. Sparks*
1
,
William Lawless*
2
,
Andres S. Arango*
1,3,4
,
Emad Tajkhorshid
3,4
,
Rutilio A. Fratti
3
1
Department of Biochemistry
,
University of Illinois at Urbana-Champaign
,
2
Department of Chemistry
,
University of South Florida
,
3
Center for Biophysics and Quantitative Biology
,
University of Illinois at Urbana-Champaign
,
4
Beckman Institute for Advanced Science and Technology
,
University of Illinois at Urbana-Champaign
Chapters
00:04
Introduction
00:49
Microscale Thermophoresis (MST) Device Preparation
01:36
Labeled MST Sample Preparation
02:24
Sample MST
02:54
Results: Representative Thermophoretic Traces
03:46
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
微尺度热泳以较低的材料成本快速获得结合常数。标记或标记的无微量级热泳是市售的;然而,无标记热泳不能使用荧光标记进行相互作用测量的多样性。我们提供用于标记热泳测量的协议。
Tags
Microscale Thermophoresis
Protein-lipid Interactions
Vam7
Phosphatidic Acid
Disassociation Constant (KD)
Biochemical Interactions
NTA-Atto 647 Dye
Thermophoretic Traces
1:1 Titration
DiC8 PA
Pharmaceutical Development
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