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使用微尺度热泳测量蛋白质-脂质相互作用
JoVE Journal
Biochemistry
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JoVE Journal Biochemistry
Use of Microscale Thermophoresis to Measure Protein-Lipid Interactions
DOI:

04:45 min

February 10, 2022

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Chapters

  • 00:04Introduction
  • 00:49Microscale Thermophoresis (MST) Device Preparation
  • 01:36Labeled MST Sample Preparation
  • 02:24Sample MST
  • 02:54Results: Representative Thermophoretic Traces
  • 03:46Conclusion

Summary

Automatic Translation

微尺度热泳以较低的材料成本快速获得结合常数。标记或标记的无微量级热泳是市售的;然而,无标记热泳不能使用荧光标记进行相互作用测量的多样性。我们提供用于标记热泳测量的协议。

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