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A Novel Stromal Fibroblast-Modulated 3D Tumor Spheroid Model for Studying Tumor-Stroma Interaction and Drug Discovery
JoVE Journal
Cancer Research
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JoVE Journal Cancer Research
A Novel Stromal Fibroblast-Modulated 3D Tumor Spheroid Model for Studying Tumor-Stroma Interaction and Drug Discovery

A Novel Stromal Fibroblast-Modulated 3D Tumor Spheroid Model for Studying Tumor-Stroma Interaction and Drug Discovery

DOI:
10.3791/60660-v

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07:20 min

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February 28, 2020

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, , , , ,
1Department of Surgery,University of Miami School of Medicine, 2Analytical Imaging Core Facility,University of Miami School of Medicine

Chapters

  • 00:04Introduction
  • 00:51Melanoma Cell and Skin Fibroblast Culture
  • 01:36Melanoma Cell and Fibroblast Fluorescent Lentivirus Transfection
  • 02:22Melanoma Cell:Fibroblast Co-Culture and Melanoma Cell Solo Culture
  • 03:25Live Cell Time-Lapse Imaging
  • 04:22Confocal Microscopic 3D Movie Generation
  • 04:59Results: Representative 3D Tumor Spheroid Formation and Analysis
  • 06:32Conclusion

Summary

Automatic Translation

Automatic Translation

A novel three-dimensional spheroid model based on the heterotypic interaction of tumor cells and stromal fibroblasts is established. Here, we present coculture of tumor cells and stromal fibroblasts, time-lapse imaging, and confocal microscopy to visualize the formation of spheroids. This three-dimensional model offers a pertinent platform to study tumor-stroma interactions and test cancer therapeutics.

Tags

Keywords: 3D Tumor SpheroidTumor-stroma InteractionDrug DiscoveryMelanomaFibroblastCo-cultureGFP Lentivirus2D Cell Cluster

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