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JoVE Journal
Immunology and Infection

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Production of High-Titer Infectious Influenza Pseudotyped Particles with Envelope Glycoproteins from Highly Pathogenic H5N1 and Avian H7N9 Viruses
 

Production of High-Titer Infectious Influenza Pseudotyped Particles with Envelope Glycoproteins from Highly Pathogenic H5N1 and Avian H7N9 Viruses

Article DOI: 10.3791/60663-v 08:10 min January 15th, 2020
January 15th, 2020

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Summary

This protocol describes an experimental process to produce high-titer infectious viral pseudotyped particles (pp) with envelope glycoproteins from two influenza A strains and how to determine their infectivity. This protocol is highly adaptable to develop pps of any other type of enveloped viruses with different envelope glycoproteins.

Tags

Keywords: Influenza Pseudotyped Particles Viral Envelope Glycoproteins H5N1 H7N9 BSL-2 Transfection QRT-PCR Virus Reassortment Cell Culture Incubation Harvest Quantification Benzonase Nuclease
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