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The CryoAPEX Method for Electron Microscopy Analysis of Membrane Protein Localization Within Ultrastructurally-Preserved Cells
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The CryoAPEX Method for Electron Microscopy Analysis of Membrane Protein Localization Within Ultrastructurally-Preserved Cells

The CryoAPEX Method for Electron Microscopy Analysis of Membrane Protein Localization Within Ultrastructurally-Preserved Cells

DOI:
10.3791/60677-v

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11:45 min

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February 27, 2020

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, , ,
1Department of Biological Sciences,Purdue University, 2Department of Medicinal Chemistry and Molecular Pharmacology,Purdue University, 3Purdue Institute of Inflammation, Immunology and Infectious Disease,Purdue University, 4Bindley Biosciences Center,Purdue University, 5Purdue Institute of Integrative Neuroscience,Purdue University, 6Purdue Center for Cancer Research,Purdue University

Chapters

  • 00:05Title
  • 01:36Transfection, Chemical Fixation and Peroxidase Reaction
  • 03:48High Pressure Freezing
  • 04:46Freeze Substitution
  • 06:23Resin Infiltration and Embedding
  • 08:11Sectioning and TEM Imaging
  • 09:17Results: TEM Images
  • 11:07Conclusion

Summary

Automatic Translation

Automatic Translation

This protocol describes the cryoAPEX method, in which an APEX2-tagged membrane protein can be localized by transmission electron microscopy within optimally-preserved cell ultrastructure.

Tags

Keywords: CryoAPEXElectron MicroscopyMembrane Protein LocalizationUltrastructural PreservationAPEX2CryofixationFreeze SubstitutionVirus ReplicationBacterial ToxinGolgi Fragmentation

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