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JoVE Journal
Biochemistry

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Stepwise Dosing Protocol for Increased Throughput in Label-Free Impedance-Based GPCR Assays
 

Stepwise Dosing Protocol for Increased Throughput in Label-Free Impedance-Based GPCR Assays

Article doi: 10.3791/60686
February 21st, 2020

Summary February 21st, 2020

This protocol demonstrates real-time recording of full dose-response relationships for agonist-induced GPCR activation from a single cell layer grown on a single microelectrode using label-free impedance measurements. The new dosing scheme significantly increases throughput without loss in time resolution.

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