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Journal / Immunology and Infection / Anvendelse af Live Cell Imaging og Cryo-Electron…
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Immunology and Infection

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JoVE Journal Immunology and Infection
Anvendelse af Live Cell Imaging og Cryo-Electron Tomography at løse Spatiotemporal Funktioner i Legionella pneumophila Dot / Icm Sekresion System
Isolation and Activation of Murine Lymphocytes
Journal (Immunology and Infection)
Isolation and Activation of Murine Lymphocytes
Static Adhesion Assay for the Study of Integrin Activation in T Lymphocytes
Journal (Immunology and Infection)
Static Adhesion Assay for the Study of Integrin Activation in T Lymphocytes
 
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Anvendelse af Live Cell Imaging og Cryo-Electron Tomography at løse Spatiotemporal Funktioner i Legionella pneumophila Dot / Icm Sekresion System

Article DOI: 10.3791/60693-v • 09:12 min • March 10th, 2020
March 10th, 2020 •
1, 1,2, 3, 1,2, 1
1Department of Microbial Pathogenesis, Boyer Center for Molecular Medicine, Yale University School of Medicine, 2Microbial Sciences Institute, Yale University, 3Department of Microbiology and Molecular Genetics, McGovern Medical School, The University of Texas Health Science Center at Houston

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Billeddannelse af bakterieceller er en ny systembiologitilgang, der fokuserer på at definere statiske og dynamiske processer, der dikterer funktionen af store makromolekylære maskiner. Her er integration af kvantitative levende celle imaging og kryo-elektron tomografi bruges til at studere Legionella pneumophila type IV sekresion system arkitektur og funktioner.

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Immunologi og infektion Problem 157 Legionella pneumophila,Legionærsyge' sygdom luftvejsinfektion intracellulære bakterielle patogener type IV sekredionssystem Dot/Icm sekreance system levende celle imaging spatiotemporal dynamik kvantitativ fluorescens mikroskopi kryoelektron tomografi cellulære strukturer allelic udveksling
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